Trymer alpha atp-03
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Trymer alpha atp-03
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Web10 µL kinase reactions using a serial dilution of kinase were performed in the presence of 10 µM ATP and the substrate and buffer ... 100 mM NaCl, 0.03% CHAPS PI4K Buffer = 20 mM Tris pH 7.5, 5 mM MgCl2, 0.5 mM EGTA, 0.4% Triton X-100 A B For lipid kinases, 10 µL kinase reactions with variable amounts of inhibitor were performed as in Figure 5.
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