Texas red phalloidin
WebAug 30, 2024 · Actin was stained with Texas-Red-phalloidin (red). Insets magnify the indicated area. Scale bar is 10 μm. (F) Fluorescence microscopy of cells expressing Emerald-vinculin (green) and Apple-actin (purple). Cells were infected with WT Salmonella, and still images taken from a live movie at 0, 6, 13, and 19 minutes post infection. Scale … WebC. DAPI/Texas Red-Phalloidin solution: DAPI is a conjugated fluorophore that binds to another molecule before fluorescing. DAPI binds to strongly enriched A-T parts of the cell, therefore it binds to the DNA and lights the nucleus blue. The Texas Red-Phalloidin binds to the phalloidin on actin and then it fluoresces red.
Texas red phalloidin
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WebMay 15, 2006 · The tagged CA domain colocalizes extensively with Texas Red–labeled Arp3, as shown by the merger of fluorescent signals. (J) Phalloidin staining of WASp-CA–expressing cells confirms disruption of actin filaments, which also failed to colocalize with the EGFP-CA fragment (data not shown). WebPhalloidin, Alexa Fluor ™ Plus 647 Phalloidin, and Alexa Fluor Plus 750 Phalloidin. Note: For a complete list of fluorescent or biotinylated phalloidin conjugates, see Table 1 on page 5. Contents and storage Contents Cat. No. Amount[1] Storage[2] Fluorescent phalloidin See Table 1 on page 5. 1 vial of lyophilized solids (300 units/assay[3])
WebNov 9, 2024 · Typically, phalloidin is used conjugated to a fluorescent dye, such as FITC, Rhodamine, TRITC or similar dyes, such as Alexa Fluor® 488 or iFluor 488. Phalloidin can … WebTo visualize actin filaments, cells were stained with Texas Red-conjugated phalloidin diluted in the blocking solution. The immunostained samples were visualized using a Nikon Eclipse TE300 inverted microscope connected to a Spot RT monochrome digital camera and image processor (Diagnostic Instruments, Sterling Heights, MI).
WebTexas Red (and Texas Red-X) dye products. We offer a broad range of Texas Red and Texas Red-X dye products including secondary antibodies, labeling kits, bioconjugates, and … WebApr 12, 2024 · Actin was visualised with Texas-Red Phalloidin (Red). Scale bar is 2 µm (D) Average percentage of S. Tm associated with an actin-rich ruffle. WT or ∆N-WASP MEFs were pre-infected with unstained WT S. Tm (WT) or treated with PBS as a control (PBS), then infected for 5 minutes with WT S. Tm pre-stained with Alexa-Fluor 350. ...
WebDescription. FluoCells prepared slide #2 contains bovine pulmonary artery endothelial cells (BPAEC). Texas Red-X phalloidin is labeling the F-actin, anti—bovine α-tubulin mouse monoclonal 236-10501 in conjunction with BODIPY FL goat anti—mouse IgG antibody is labeling microtubules, and DAPI is labeling the nucleus.
WebPhalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications … hot oil heaters for asphalt plantsWebAdd Phalloidin-iFluor™ 594 Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under microscope with TRITC or Texas Red filter Important Warm the vial to room temperature and centrifuge briefly before opening. Storage and Handling Conditions lindsey cowan paul terryWebMay 20, 2024 · Actin (red) was stained with Texas Red-phalloidin, and nuclei (blue) stained with DAPI. Cells were imaged at magnification ×63, and shown are projections of z-stacks generated with Volocity. D) Volume and E) Percent co-localization of Ltp1 or Php1 in the nuclear region of the images in (C) * p< 0.05, **** p < 0.001 compared to NI. lindsey courtneyWebJun 1, 2024 · Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. Selectively stains F-actin … lindsey coxWebRhodamine Phalloidin is conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC) (Figure 5). Alexa Fluor 546, Alexa Fluor 568, Alexa Fluor 594, and Texas Red-X (Figure 6) phalloidins are available … hot oil in eye redditWebTexas Red™-X phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Texas Red™-X phalloidin staining is fully … Texas Red™-X phalloidin is a high-affinity F-actin probe conjugated to our bright, … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes hot oil horsesWebOct 1, 1999 · For visualization of intracellular F-actin organization, the cells were probed with Texas Red-conjugated phalloidin. For detection of nontagged CPD, the samples were incubated with the anti-CPD antibody, followed by an FITC-conjugated secondary antibody. Fluorescent images were visualized using a confocal microscope (MRC1024; Bio-Rad). … hot oil in hair dryer