Phospho 4ebp1 antibody

Author: pfnf

August undefined, 2024

WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the … Webp-4E-BP1 Antibody (62.Ser 65): sc-293124. Western blot analysis of 4E-BP1 phosphorylation in HeLa (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates. Description Gene Info Datasheets and Protocols The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA.

4EBP1 Antibodies - Novus Biologicals

WebThe Phospho-4EBP1 (Thr37/46) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. WebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … high level language to machine language

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb 2855S from

WebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E … WebAnti p-eIF4EBP1 (Ser65/Thr70) antibody, Anti BP1 antibody, Anti BP 1 antibody, Anti eIF4EBP1 (phospho Ser65/Thr70) antibody, Anti PHAS I antibody, Anti 4EBP1 antibody, Anti PHASI antibody, Anti 4E-BP1 antibody, Anti eIF4E-binding protein 1 antibody, Anti eIF4EBP1 (phospho S65/Th70) antibody, Anti 4E BP1 antibody, Anti eIF4E binding protein 1 … WebInvitrogen Anti-Phospho-4EBP1 (Thr37, Thr46) Polyclonal, Catalog # PA5-114544. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) (IHC (PFA)) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL). high level languages

Purified anti-4EBP1 Phospho (Thr70) Antibody - BioLegend

Anti-eIF4EBP1 antibody (ab2606) Abcam

WebAnti-4E-BP1 (phospho Ser65 + Thr70) Antibody (A255095) Overview Specifications Images £240 View Datasheet Rabbit polyclonal antibody to 4E-BP1 (phospho Ser65 + Thr70) for WB, IHC and ICC/IF. 100% Guarantee Price Match Guarantee Product Size: 100µl 200µl Quantity: Add To Cart Shipping Information Contact Us Guarantees Specifications Name WebJun 12, 2014 · Total 4EBP1 antibody detects both phosphorylated and non-phosphorylated protein. Samples were treated with lambda-phosphatase or reaction buffer alone and decrease in phosphorylation was noted. X-axis represents iso-electric pH and y-axis represents luminescence units. high level language vs machine languageWebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体， ... high level layered cloud that produces a halo

"WebOur 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the 4EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. " - Phospho 4ebp1 antibody

Phospho 4ebp1 antibody

Phospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody

WebMA Beigh's Publication in Biomed Res Int....Sigma unless otherwise indicated. Anti-S6K antibody (Santa Cruz Biotechnologies Inc), Anti-S6K (Polyclonal-Genscript), Anti-Erk1, Anti-phospho-Erk, Anti-4EBP1, Anti-phospho- 4EBP1-T70 and Anti-phospho-S6K Thr412 antibodies were obtained from Cell Signaling... WebMar 30, 2016 · When 4EBP1, an eIF4E-binding protein, is active (non-p-4EBP1), it binds to eIF4E and hinder formation of the initiation complex and then translation is blocked, favoring apoptosis....

Did you know?

Web实验用到的供体是Thr46（铽标记的anti-phospho-4EBP1），受体是绿色荧光标记的4EBP1，是mTOR下游信号通路的蛋白，有活性的mTOR能磷酸化4EBP1，使之与供体结合。如果mTOR活性受到抑制，供体受体距离不能达到荧光能量转移的要求，则在515nm处与485nm处的信号比值增大。 WebView Rabbit Polyclonal anti-4EBP1 [p Thr46, p Thr37] Antibody (NBP3-13169). Validated Applications: WB. Validated Species: Human, Mouse, Rat + more. Validated by: Biological Strategies, Genetic Strategies.

WebPhospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View all (63) 4EBP1 antibodies Add To Cart Save to list Performance Guarantee Datasheet Protocols Questions & Answers Tech Support Antibody Testing Data (1) Web肢体畸形相关蛋白FHOD1抗体为客户提供优质的产品服务及最新的产品信息和最优惠的价格，购买时注意检查收货地址，我们不断丰富产品的种类，且全国各网点均可安全、快速配送上门，为您提供超速满意的产品服务，强烈建议广大客户购买前联系我们，确认产品信息及库存状况。肢体畸形相关蛋白 ...

WebFigure 3: Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (AF2308) Western blot analysis of extracts from 293 cells treated with 20% FBS , using Phospho-4EBP1(Ser65/Thr70) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide. WebListed below are anti-4EBP1 antibodies from multiple suppliers. 4EBP1 is a reported alias name for the human gene EIF4EBP1, or 'eukaryotic translation initiation factor 4E binding protein 1'. ... Immunotag™ Phospho-4EBP1(Ser65/Thr70) Antibody. Applications: WB, IHC; Reactivity: Hu, Ms, Rt; Conjugate/Tag: Unconjugated, Biotin, FITC, HRP, Alexa ...

WebFeb 10, 2024 · Cell Signaling Technology's Phospho-4E-BP1 (Thr70) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.

WebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ... high level languages areWebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species … high level languages in computerWebEach 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. high level led brake lightWeb(orb502277) 4EBP1 (phospho-Ser65/Thr70) antibody (Biotin) - Biorbyt - CiteAb A Biotin-conjugated rabbit polyclonal antibody, raised against Eukaryotic translation initiation factor 4E-binding protein 1 (Mouse), supplied by Biorbyt. high level led brake lightsWebAnti-eIF4EBP1 (phospho T36) antibody See all eIF4EBP1 primary antibodies Description Rabbit polyclonal to eIF4EBP1 (phospho T36) Host species Rabbit Specificity ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. high level low income housingWebThe Simple Western TM antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. high level lingueeWebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence. high level logic