High-throughput screens for eef-2 kinase

Author: wbds

August undefined, 2024

WebJun 10, 2014 · High-throughput screening platforms have proved beneficial for protein kinase drug discovery and development, as large compound libraries can be screened with ease and compound potency and selectivity across a subset of the kinome can be determined [13], [14]. WebThese yeast-based methods range from HTP screens to identify novel hits against promising parasite kinase targets to the identification of potential antiparasitic kinase inhibitors extracted from databases of yeast chemical genetic screens. Keywords: Drug discovery; Drug screening; High-throughput; Kinase inhibitors; Neglected diseases; Yeast.

Yeast-based high-throughput screens for discovery of kinase …

WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of … WebHere, we describe the purification of the protein kinase, eEF-2K, and the utilization of three biochemical assays in the course of identifying small molecules that block its enzymatic … iowa small claims statute

High-throughput screens for eEF-2 kinase - Houston Methodist …

WebMay 1, 2014 · The activity of eEF-2, the main eEF involved in the translocation step of elongation, can be inhibited by the eukaryotic elongation factor 2 kinase (eEF2K) (Ma, 2024). eEF2K is an... WebSep 18, 2024 · A high-throughput screen (kinase reaction performed at room temperature) revealed the compound TCMDS-135051 as a highly selective and specific Pf CLK3 kinase inhibitor. This was confirmed in vivo by the finding that parasite strains that developed resistance against TCMDS-135051 acquired mutations in Pf CLK3. WebMay 1, 2016 · Eukaryotic elongation factor 2 kinase (eEF2K) inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was … iowa small claims verification of account

Medicines Free Full-Text Discovery of Novel eEF2K Inhibitors …

Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for ...

WebJan 24, 2014 · Using this high-throughput assay, we screened more than 7000 compounds and discovered additional small molecules that inhibit the ATM kinase and further validated these hits by secondary assays. Introduction WebNov 26, 2007 · Kinases are important drug discovery targets for a wide variety of therapeutic indications; consequently, the measurement of kinase activity remains a common high-throughput screening (HTS) application. Recently, enzyme-coupled luciferase-kinase (LK) format assays have been introduced. iowa small claims service of processWebJan 25, 2016 · Eukaryotic elongation factor 2 kinase (eEF2K) is an unusual protein kinase that regulates the elongation stage of protein synthesis by phosphorylating and inhibiting … iowa small claims threshold

"WebOct 26, 2015 · High-Throughput Screens for eEF-2 Kinase. Article. Sep 2013; Ramakrishna Edupuganti; Ashwini K Devkota; Mangalika Warthaka; Kevin Dalby; eEF-2 kinase is a potential therapeutic target for breast ... " - High-throughput screens for eef-2 kinase

High-throughput screens for eef-2 kinase

Development of a Cell-Based, High-Throughput Screening Assay …

WebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput … WebMay 21, 2016 · Eukaryotic elongation factor 2 kinase (eEF2K) inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was …

Did you know?

WebJul 13, 2024 · In the same study, the identification of a selective but weak inhibitor of eEF2K (IC 50 = 0.28 μM) called A-484954 through a high-throughput screen was also reported … WebHigh-throughput screens for eEF-2 kinase (Q35002039) From Wikidata. Jump to navigation Jump to search. scientific article. edit. Language Label Description Also known as; English: High-throughput screens for eEF-2 kinase. scientific article. Statements. instance of. scholarly article. 1 reference. stated in.

WebEnter the email address you signed up with and we'll email you a reset link. WebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput …

WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput assay systems may expedite the process. Two high-throughput assays are described for eEF-2K using recombinant, tag-free enzyme purified from bacteria. WebAug 24, 2024 · The High-throughput screening method screens millions of chemical and biological compounds in a short interval of time. It is an automated process and screens many biological or chemical compounds for their therapeutic potential. ... As an example, the high-throughput screening process successfully identified a potent pan-SRC kinase …

WebJul 15, 2014 · Eukaryotic elongation factor-2 kinase (eEF2K), encoded by the EEF2K gene, is well-known to be a Ca 2+ /calmodulin (CaM)-dependent kinase which can negatively modulate protein synthesis. It is highly conserved among eukaryotes from mammals to invertebrates, of which human and mouse may have 99 % overall amino acid identity.

WebWe report here the development and optimization of a simple 384-well colorimetric assay to measure H(2)O(2) generated by the redox cycling of compounds incubated with reducing agents in high-throughput screening (HTS) assay buffers. The phenol red-horseradish peroxidase (HRP) assay readily detected H(2)O(2) either added exogenously or generated … iowa small estate affidavit pdfWebMay 21, 2016 · Search worldwide, life-sciences literature Search. Advanced Search iowa small estate lawWebEvidence that elongation factor 2 kinase (eEF-2K) has potential as a target for anticancer therapy and possibly for the treatment of depression is emerging. Here the steady-state … iowa small engine carroll iaWebApplication of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening Authors Clint D J … open eyes cream reviewsWebSep 27, 2013 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … open f1 and f2 using cWebNov 1, 2016 · Purified human eEF2K was obtained from an expression system and a luminescence-based high-throughput screening (HTS) assay was developed using MH-1 … open eyes in frenchWebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of … iowa small estate form